ABS 026-05-02

antibody from Invitrogen Antibodies

Targeting: CALCA CALC1, CGRP, CGRP-alpha

Immunohistochemistry (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

ABS 026-05-02

Provider

Invitrogen Antibodies

Product name

CGRP Monoclonal Antibody (5)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Description

ABS 026-05-02 detects CGRP alpha in human and rat samples. ABS 026-05-02 binds human alpha-CGRP. Based on inhibition ELISA, this antibody shows full cross-reactivity with rat alpha-CGRP and about about 50% cross-reactivity with human beta-CGRP. Cross-reactivity with coated rat alpha-CGRP is low (8%) and there is no cross-reaction with human amylin.

Antibody clone number

5

Concentration

1.02 mg/mL

References

Submitted references

UEG Week 2020 Poster Presentations.

United European gastroenterology journal 2020 Oct;8(8_suppl):144-887

---

A novel role for the extracellular matrix glycoprotein-Tenascin-X in gastric function.

Aktar R, Peiris M, Fikree A, Eaton S, Kritas S, Kentish SJ, Araujo EJA, Bacarin C, Page AJ, Voermans NC, Aziz Q, Blackshaw LA
The Journal of physiology 2019 Mar;597(6):1503-1515

---

The extracellular matrix glycoprotein tenascin-X regulates peripheral sensory and motor neurones.

Aktar R, Peiris M, Fikree A, Cibert-Goton V, Walmsley M, Tough IR, Watanabe P, Araujo EJA, Mohammed SD, Delalande JM, Bulmer DC, Scott SM, Cox HM, Voermans NC, Aziz Q, Blackshaw LA
The Journal of physiology 2018 Sep;596(17):4237-4251

---

Superficial Esophageal Mucosal Afferent Nerves May Contribute to Reflux Hypersensitivity in Nonerosive Reflux Disease.

Woodland P, Shen Ooi JL, Grassi F, Nikaki K, Lee C, Evans JA, Koukias N, Triantos C, McDonald SA, Peiris M, Aktar R, Blackshaw LA, Sifrim D
Gastroenterology 2017 Nov;153(5):1230-1239

---

Distinct afferent innervation patterns within the human proximal and distal esophageal mucosa.

Woodland P, Aktar R, Mthunzi E, Lee C, Peiris M, Preston SL, Blackshaw LA, Sifrim D
American journal of physiology. Gastrointestinal and liver physiology 2015 Mar 15;308(6):G525-31

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemical analysis of CGRP alpha in paraffin-embedded rat tissue using a CGRP alpha monoclonal antibody (Product # ABS 026-05-02) after overnight incubation at a dilution of 1:400 in 1% BSA/PBS.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 5. Representative immunostaining for CGRP and protein gene product (PGP) in the proximal ( A-C ) and distal ( D-F ) esophageal mucosa.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig. 4. Transverse sections of esophageal biopsies showing CGRP-immunoreactivity (lumen uppermost). A : example of a proximal esophageal biopsy, with CGRP-immunoreactive fibers a few cell layers from the luminal surface. B : example of a distal esophageal biopsy, demonstrating a nerve fiber in the basal epithelium (most superficial part of fiber 26 cell layers from the lumen). Images magnified x40. Scale bar represents 100 mum.